The Production of Second-generation Bioethanol from Lignocellulosic Biomass using Two Strains of Sacharomyces cerevisiae

Concerns about first generation bioethanol's impact on the food chain and biodiversity have shifted research to second (2G) bioethanol technologies. The 2G-bioethanol is made from lignocellulosic biomass, which more sustainable does not harm security or environment. This production process uses non-food crops, crop residues, wood wastes, such as chips, skins, pulp fruit pressing. present study examines potential of three biomass residues: corn cob, husk, stem, well their physiochemical mineral composition before after fermentation. Before fermentation, waste samples were hydrolyzed into sugar monomer hydrolysate was fermented separately produce for five days at 282oC using two Saccharomyces cerevisiae strains: typed yeast ATCC 3585 Baker's 204508/S288c. At one-day intervals, pH, simple ethanol measured. ANOVA used find significant differences between investigated organisms. results showed that 35858 produces than other strain (20.25±0.63). Corn cob also produced stem husk. During yeasts outperformed in reducing sugar, specific gravity. Average dry cell mass (ADM) 204508/S288c 1.82±0.07 1.98±0.03, respectively. According proximate composition, fermentation lost over 50% waste's nutrients (ash), while recovering minerals (nitrogen, phosphorus, potassium). ability strains significantly different p value ≤ 0.05.